Optimizing Collagenase, Fetal Bovine Serum, and Insulin to Isolate, Proliferate, and Differentiate Rat Preadipocyte in Vitro  

Oleh:

Widodo, Edwin ; Indra, M. Rasjad ; Satuman

Jenis: Article from Journal - ilmiah nasional - tidak terakreditasi DIKTI
Dalam koleksi: Jurnal Kedokteran YARSI vol. 16 no. 03 (Sep. 2008), page 150-156.
Topik: Obesity; Culture Media; Implant Tissue; Adipocyte Precursor; Proliferation

Ketersediaan

Perpustakaan PKPM Nomor Panggil: J51 Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada	Lihat Detail Induk
Perpustakaan FK Nomor Panggil: J26.K Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada	Lihat Detail Induk

Isi artikel

Background the aim of this study is to develop a method for optimal in vitro proliferation and differentiation of rat adipocyte. Methods Preadipocyte were isolated from omentum of rattus norvegicus wistar using collagenase type I dan II (sigma). Cells were cultured in M199 culture containing either 0%, 8% or 10% fetal bovine serum (FBS). insulin were added into the media once the cells attached to the culture plate proliferating and differntiated cells were counted and analysed by using oil red o dan hematoxylen (HE) staining. Results this study demonstrated that adipocyte could be isolated using collagenase type I but not type II. the addition of 10% FBS significantly increased the number of preadipocyte and differentiation of adipocyte more than those of 8% FBS and without FBS. the timing of FBS addition was best performed on day 8 using 10% FBS. specific adipocyte staining using oil red O revealed that there were core lipids in mature adipocyte. Conclusions collagenase tipe I could be used to isolate preadipocyte cells. suppementation of culture media with 8-10% FBS could enhance the in vitro proliferation of preadipocyte. standard media M199 containing 10% FBS and insulin may provide an environment to differentiate preadipocyte specifically into adipocyte in vitro.

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