Photoelectrohemical Detection Od Dengue-Related Oligonucleotide sequemce Using Anthocyanin As An Intercalating Agent And Electrochiromic Material  

Oleh:

Ibrahim, Anwar Soefi ; Kusmardi

Jenis: Article from Journal - ilmiah nasional - terakreditasi DIKTI
Dalam koleksi: Makara Seri Kesehatan vol. 14 no. 2 (Dec. 2010), page 70-74.
Topik: Anthocyamin Extract; Dengue-related Oligonucleotide; Photoelectrochemical

Ketersediaan

Perpustakaan Pusat (Semanggi) Nomor Panggil: MM75.2 Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada

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Isi artikel

World Health Organization (WHO) presupposes a confirmation of dengue virus infection diagnosis with two criteria, i.e. clinical and laboratory criteria. One of the basic methods used by most laboratories to diagnose dengue virus is to detect oligonucleotide sequences using a DNA amplification technique. In this research, the measurement of dengue-related oligonucleotide was conducted by photoelectrochemical method. The presece of oligonucleotide sequemce in target DNA can be detected by DNA probe that is immobilized on TiO2 electrode. The DNA hybrid is then bound to electrochromic substance like anthocyanin that generates current when it is subjected to light. The photocurrent is directly proportional to the number of target DNA. The aim of this research is to photoelectrochemical system that has sensitivity and high responsiveness toward the change in oligonucleotide concentration, especially the applicability of anthocyanin as a electrohromic substance and intercalating agent. Linearity (R2) generated from the change of current in response to concentration changes of target DNA (in the concentration range of 0.75-3.00 nM) is 0.9611. Thus, this method has the potential to be developed to detect the presence of dengue virus in biological sample.

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