Effects of polybrominated diphenyl ethers on steroidogenesis in rat Leydig cells  

Oleh:

Kai-Lee, Wang ; Shih-Min, Hsia ; I.-Fang, Mao ; Mei-Lien, Chen ; Shyi-Wu, Wang ; Wang, Paulus S.

Jenis: Article from Journal - ilmiah internasional
Dalam koleksi: Human Reproduction vol. 26 no. 08 (Aug. 2011), page 2209-2217.
Topik: REPRODUCTIVE ENDOCRINOLOGY; Polybrominated Diphenyl Ethers Leydig Cells Steroidogenesis Cyclic AMP Testosterone

Ketersediaan

Perpustakaan FK Nomor Panggil: H07.K.2011.01 Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada

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Isi artikel

BACKGROUND Polybrominated diphenyl ethers (PBDEs) are brominated flame retardants that have been defined as major environmental pollutants. While previous studies have found that PBDEs may enhance the levels of sex-steroid hormones, their effects on testosterone secretion from rat Leydig cells are unclear. This study investigated the effects and mechanisms of PBDE-710, a mixture of tetra- and penta-PBDEs, on testosterone biosynthesis in rat Leydig cells. METHODS Leydig cells from adult male rats were challenged with different concentrations of PBDE-710 (0.5–15 ng/ml) to evaluate the effects on testosterone steroidogenesis. Concentrations of testosterone and of cAMP and pregnenolone in medium were measured by radioimmunoassay (RIA) and by enzyme-linked immunosorbent assay, respectively. Nuclear translocation of protein kinase A a (PKAa) was determined by immunofluorence assay and western blot assay, and the mRNA expression of steroidogenic acute regulatory protein (StAR) was analyzed by quantitative real-time polymerase chain reaction. RESULTS In this in vitro study, PBDE-710 (5 or 15 ng/ml) increased basal testosterone secretion and cAMP production by 3- and 2-fold, respectively. The stimulatory effect was abolished by adenylyl cyclase inhibitor. Enzyme activity of CYP11A1, as determined by the pregnenolone concentration, was stimulated by PBDE-710 treatment. Furthermore, nuclear translocation of PKAa was increased by 20% and StAR gene expression was elevated by 4-fold after PBDE-710 treatment. CONCLUSIONS These results suggest that low concentrations of PBDE-710 could stimulate testosterone secretion by acting directly on Leydig cells to activate the cAMP pathway and increase expression of StAR.

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