Background Indonesia is included as 10 countries with largest prevalence of diabetes, which is 6.2% of population. Urinary tract infection (UTI) patients with diabetes mellitus (DM) are almost twice more than without DM. Variative UTI-causing organisms complicate antibiotic’s selection for UTI thus provoke importance in pattern of aerobic bacteria causing UTI and its sensitivity to antibiotic. Objective To know pattern of aerobic bacteria causing UTI and its sensitivity to antibiotic in patients with diabetes mellitus at Atma Jaya hospital. Methodology This research is descriptive research with cross-sectional retrospective design. Researcher took urine’s bacterial cultural data with significant bacteruria in diabetic patients with UTI at Atma Jaya hospital. Research was done in microbiology laboratory of faculty of medicine of Atma Jaya uiversity using data in 2014-2016 analyzed with WHONET v. 5.6. Results and Conclusions Out of 42 isolates, Escherichia coli (61,9%), Klebsiella pneumoniae (9,52%), and Acinetobacter baumanni (9,52%) were the most frequent bacteria isolated among UTI cases on diabetic patients. Fosfomycin (92,3%) was the most sensitive antibiotic for Escherichia coli. Cefoxitin, cefpodoxim, amikacin, gentamycin, and imipenem (100%) were the most sensitive antibiotics for Klebsiella pneumoniae. Acinetobacter baumanni is the most sensitive to carbapenems (100%). Multidrug resistance were found in aerobic bacteria as etiology of UTI in patients with DM |