Background: Coronary heart disease (CHD) is the leading cause of mortality worldwide. Hypercholesterolemia is well known as one of the risk factors. The
pathogenesis of CHD is athersoclerosis, which started with plaque formation by low
density lipoprotein and oxidative stress. Oxidative stress increase malondialdehyde
(MDA) levels by fatty acid membrane cell peroxidation. Moringa Oleifera is a plant
that rich in flavaonoid, phenolic, alkaloid, and vitamins. These compounds act as
antioxidants and have a hypocholesterolemic effect that can counteract the effects of
hypercholesterolemia
Methods: This experiment is post test only with control group design. Samples are
male Sprague Dawley rats that are fed with high cholesterol diet and aqueous
extract with 100mg/bw, 150mg/bw, and 200mg/bw dose for 21 days. Plasma MDA
levels and total cholesterol levels are quantified with TBARS and CHOD PAP
method respectively.
Result: Compared to control, there were differences in mean total cholesterol levels
by 12%, 15%, and 20% in groups that are given with aqueous extract but aren’t
significant. Meanwhile, mean MDA levels differed significantly in groups that are
given with 150 and 200mg/bw dose of extract (P<0,05), but not in 100mg/bw.
Conclusion: Various dose of moringa oleifera aqueous leaf extract didn’t cause any
significant difference in total cholesterol level, but at 150mg/bw and 200mg/bw,
these extracts cause significant difference in plasma MDA levels. |