Application Of Hydroxyapatite In Protein Purification  

Oleh:

Witarto, Arief Budi

Jenis: Article from Journal - ilmiah nasional - terakreditasi DIKTI
Dalam koleksi: Makara Seri Sains vol. 13 no. 2 (Nov. 2009), page 134-140.
Topik: Hydroxyapatite; Protein Purification; Wet Method

Ketersediaan

Perpustakaan Pusat (Semanggi) Nomor Panggil: MM65 Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada

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Isi artikel

The precursors , Na2HPO4.2H2O and CaCl2.2H2O are used for synhesizing pure hydroxyapatite which less of carbonate content. The high temperature of sintering, about 700degreeC of temperature, is treated to minimize the carbonate group on hydroxyapatite surface. Carbonate content of hydroxyapatite which is sintered in 700degreeC is less than 110degreeC. It indicates an increasing temparature of sintering will increase crystallinity and decrease carbonate content of hydroxyapatite. This method gave better way to resulting hydroxyapatite crystal. The patterns of band of SDS PAGE in resulting of Protein Purification by DEAE matrix is also appear in using hydroxyapatite matrix. The other band also appear in purification by hydroxyapatite matrix, showed that hydroxyapatite not anly has DEADE matrix characteristic as anion exchange but also has kation exchange characteristic. These patterns proof that hydroxyapatite could be used repeatedly more than one hundred times without contamination.

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