Thyroid-stimulating hormone receptor and thyroid hormone receptors are involved in human endometrial physiology  

Oleh:

Aghajanova, Lusine ; Stavreus-Evers, Anneli ; Lindeberg, Maria ; Landgren, Britt-Marie ; Sparre, Lottie Skjoldebrand ; Hovatta, Outi

Jenis: Article from Journal - ilmiah internasional
Dalam koleksi: Fertility and Sterility (keterangan: ada di ClinicalKey) vol. 95 no. 01 (Jan. 2011), page 230-237.
Topik: Deiodinases; human endometrium; thyroid hormone receptor; thyroid hormones; TSH receptor

Ketersediaan

Perpustakaan FK Nomor Panggil: F02.K.2011.02 Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada

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Isi artikel

Objective To study the expression, distribution, and function of thyroid-stimulating hormone receptor (TSHR) and thyroid hormone receptors (TR) a1, a2, and ß1 in human endometrium. Design Experimental clinical study. Setting University hospital. Patient(s) 31 fertile women. Intervention(s) Endometrial biopsy samples obtained throughout the menstrual cycle. Main Outcome Measure(s) Real-time reverse transcriptase polymerase chain reaction, immunohistochemistry and Western blot to study the expression of TSHR, TRa1, TRa2, and TRß1 messenger RNA (mRNA) and proteins in human endometrium. Result(s) We found TSHR, TRa1, TRa2 and TRß1 mRNA and proteins expressed in human endometrium. Immunostaining for TSHR in the luminal epithelium and TRa1 and ß1 in the glandular and luminal epithelium increased statistically significantly on luteinizing hormone (LH) days 6 to 9, coinciding with appearance of pinopodes. Endometrial stromal and Ishikawa cells expressed mRNA for TSHR, TR, and iodothyronine deiodinases 1–3. After 48 hours, TSH significantly increased leukemia inhibitory factor (LIF) and LIF receptor (LIFR) messenger RNA (mRNA) in endometrial stromal cells, but decreased their expression in Ishikawa cells. Glucose transporter 1 mRNA was up-regulated by TSH in Ishikawa cells. We found that TSH statistically significantly increased secretion of free triiodothyronine (T3) and total thyroxin (T4) by Ishikawa cells compared with nonstimulated cells. Conclusion(s) Thyroid hormones are directly involved in endometrial physiology.

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