Bioenergetic Analysisof FLAG Tagged-Subunit 8 of Saccharomyces cervisiae Mitochondrial ATP Synthase  

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Artika, I Made

Jenis: Article from Journal - ilmiah nasional - terakreditasi DIKTI
Dalam koleksi: Microbiology Indonesia vol. 4 no. 3 (Dec. 2010), page 108-112.
Topik: bioenergetic; ATP synthase; mitochondria; yeast
Fulltext: Bionergetic_Ros.pdf (75.94KB)

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Perpustakaan Pusat (Semanggi) Nomor Panggil: MM78 Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada

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Isi artikel

The majority of cellular energy in the form of adenosine triphosphate (ATP) is synthesized by the F1F6-ATP synthase. The yeast mitochondrial F1F6-ATP synthase is a multisubunit complex that contains at least 17 different subunits grouped into F1 and F6 sectors. Subunit 8 of yeast mitochondrial ATP synthase is a hydophobic protein of 48 amino acids encoded by the mitochondrial ATP8 gene. Subunit 8 has three distinct domains; an N-terminal domain, a central hydrophobic domain and a C-terminal domain. FLAG tag addition to the C-terminus of subunit 8 and its variants has facilitated elucidation of subunit 8's membrane topology. In order to analyze its detailed structure and function, a set of strains expressing FLAG tagged-subunit 8 and its variants were subjected to bioenergetic analysis at cellular and mitochondrial levels. Result obtained showed that the hydrophobic character of the central hydrophobic domain of subunit 8 is essential for functional coupling between F1 and F6 sectors, hence for mitochondrial ATP synthase function.

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