Protamine 1 to protamine 2 ratio correlates with dynamic aspects of DNA fragmentation in human sperm  

Oleh:

Garcia-Peiro, Agustin ; Martinez-Heredia, Juan ; Oliver-Bonet, Maria ; Abad, Carlos ; Amengual, Maria Jose

Jenis: Article from Journal - ilmiah internasional
Dalam koleksi: Fertility and Sterility (keterangan: ada di ClinicalKey) vol. 95 no. 01 (Jan. 2011), page 105-109.
Topik: Spermatozoa; protamine; P1/P2 ratio; DNA integrity; infertility; DNA damage

Ketersediaan

Perpustakaan FK Nomor Panggil: F02.K.2011.02 Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada

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Isi artikel

Design P1/P2 ratio was analyzed using acid-urea polyacrylamide acid-urea gels electrophoresis (PAGE). Sperm DNA fragmentation using sperm chromatin dispersion methodology was analyzed after 0, 4, 8, and 24 hours of incubation at 37°C. Setting University medical school and hospital. Patient(s) A total of 32 human males: six with proven fertility, seven carriers of chromosome reorganizations, nine clinical varicocele patients, and ten subclinical varicocele patients. Intervention(s) None. Main Outcome Measure(s) P1/P2 ratio, sperm DNA fragmentation (SDF) and the rate of sperm DNA fragmentation (rSDF). Result(s) P1/P2 ratio correlated with SDF and rSDF. Statistical differences were detected between fertile controls and patients for the three pathologies studied. rSDF yielded information that differed from baseline SDF. No differences were detected for P1/P2 ratio among patient groups, in reference to the three pathologies studied. Conclusion(s) SDF and rSDF correlates with P1/P2 ratio in human sperm, and statistical differences were detected when fertile controls were compared with three different cohorts of patients.

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