In-House RT-PCR Assay For Detection Of Human Immunodeficiency Virus Type 1 Infention  

Oleh:

Yasmon, Andi ; Ibrahim, Fera ; Bela, Budiman

Jenis: Article from Journal - ilmiah nasional - terakreditasi DIKTI
Dalam koleksi: Makara Seri Kesehatan vol. 13 no. 2 (Dec. 2009), page 96-98.
Topik: HIV-1; RT-PCR; Serologic Assay

Ketersediaan

Perpustakaan Pusat (Semanggi) Nomor Panggil: MM75.2 Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada

Lihat Detail Induk

Isi artikel

Serologic assays are commonly used for screening (ELISA) and for confirmation (Western blot) of HIV-1 infection; however, both assays have potentially yielded the false-positive or false-negative results. In this study, we developed a diagnostic RT-PCR assay as an alternative test for detection of HIV-1. Forty-six plasma specimens from highly risky groups, who visited the voluntary counseling and testing for HIV (VCT) in Sanglah Clinic of General Hospital, Denpasar, Bali, were tested by RT-PCR assay with specific primers for Pol region of HIV-1 genome. The results of the RT-PCR tests were then compared with those of serologic tests for obtaining the sensitivity and specificity of RT-PCR assay. The results of this study showed that the RT-PCR assay could detect 17 (sensitivity: 65.4%) of 26 serologically positive specimens and was unexpectedly able to detect 2 (specificity: 90%) of 20 serologically negative specimens. Thus, the RT-PCR assay developed in this study is potential to be used as alternative test, even though there are numerous aspects, particularly the sensitivity that needs to be improved in further research.

Opini Anda

Klik untuk menuliskan opini Anda tentang koleksi ini!

Kembali