Antihyperglycemic Activity Of The Mahkota Dewa Leaf Extracts As An Alpha-Glucosidase Inhibitor  

Oleh:

Sugiwati, Sri ; Setiasih, Siswati

Jenis: Article from Journal - ilmiah nasional - terakreditasi DIKTI
Dalam koleksi: Makara Seri Kesehatan vol. 13 no. 2 (Dec. 2009), page 74-78.
Topik: Alpha-Glucosidase Inhibitor; Diabetes Mellitus Type 2; Mahkota Dewa; Phaleria Macrocarpa

Ketersediaan

Perpustakaan Pusat (Semanggi) Nomor Panggil: MM75.2 Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada

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lpha-glucosidase (EC 3.2.1.20) was an carbohydrase that catalyzes the liberation of a-glucose from the nonreducing end of the diet carbohydrate. In diabetic patients, inhibition of these enzymes cause restrain of glucose absorption and decrease the postprandial hyperglycemia. The purpose of this research was to study the antihyperglycemic activity of mahkota dewa [Phaleria macrocarpa (Scheff.) Boerl.] leave extracts by inhibition test to alpha glucosidase enzyme. This research was conducted in three steps: fractionation and extraction samples with methanol, ethyl acetate, n-butanol and water, followed by phytochemistry screening and alpha-glucosidase inhibition test. The alpha-glucosidase inhibition test was performed by using alpha-glucosidase enzyme and p-nitrophenyl a-D-glucopyranoside as a substrate. The result of phytochemistry screening showed that Mahkota Dewa leaves contained class of phenolics, thanins, flavonoids, alkaloids and carbohydrates. The result of alpha-glucosidase inhibition test showed that ethyl acetate fraction extract had the highest inhibition activity with inhibition percentage at 50 ppm for old leave is 55.04% and for young leave is 56.92%. At 50 ppm, inhibition activity from the methanol extract and boiled water extract of old leave are higher than young leave with inhibition percentage of old leaves methanol extract is 14.25% and 10.97% for young leaves and for old leave boiled water extract is 10.32% and 6.85% for young leaves. For n-butanol fraction extract, inhibition activity of young leave extract (14.26%) was higher than old leave extract (9.49%).

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