Pertumbuhan dan Produksi Astaxanthin Khamir Phaffia Rhodozyma Pada Berbagai Sumber Karbon dan Jenis Cahaya  

Oleh:

Suparmi ; Nurcahyanti, Agustina Dwi Retno ; Budiyanto, Aji Wahyu ; Pratiwi, Reny ; Mahendra, Edwin ; Prasetyo, Budi

Jenis: Article from Journal - ilmiah nasional
Dalam koleksi: Mandala of Health (A Scientific Journal) vol. 04 no. 03 (Sep. 2010), page 139-146.
Topik: astaxanthin; light; fructose; glucose; cell growth; Phaffia rhodozyma

Ketersediaan

Perpustakaan FK Nomor Panggil: M50.K.01 Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada

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Today's rapidly growing demand for food color, in line with the development of food industry, medicine and cosmetics. Astaxanthin is one of the carotenoid pigments from the yeast Phaffia rhodozyma as a source of drugs to consider as well as natural dyes, considering that this pigment has a biological activity that is useful, for human health. Therefore, the growth and astaxanthin production of Phaffia rhodozyma need to be optimized. This study aimed to investigate the influence of light and medium carbon sources on growth and astaxanthin production of Phaffia rhodozyma yeast. P.rhodozyma grown in batch fermentation with glucose and fructose as carbon source and irradiated with light (500 lux) light both red and white light (polychromatic) and in dark conditions at a temperature of 27±2 o C for 5 days. Astaxanthin is produced under conditions of light and no light (dark) is extracted from biomass, identified and quantified by absorption spectra using a UV-Vis spectrophotometer. The growth rate of P. rhodozyma in dark conditions than in conditions of greater exposure to the light even though this does not seem signrficant. Production of astaxanthin by the yeast is also influenced in particular by light. P. rhodozyma produce large amounts of astaxanthin in exposure to light than in the dark. However, carotenoid production is not followed the same pattern based on carbon source. When P. rhodozyma grew under ,fermentative conditions with different carbon sources with different irradiated and polychromatic red light, speed of production of astaxanthin from the highest to the lowest in a row is the yeast that was grown with carbon sources glucose, fructose, and without source carbon.

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