Celecoxib Inhibits Beta-catenin-dependent Survival of the Human Osteosarcoma MG-63 Cell Line  

Oleh:

Xia, J-J ; Pei, L-B ; Zhuang, J-P

Jenis: Article from Journal - ilmiah internasional
Dalam koleksi: The Journal of International Medical Research vol. 38 no. 04 (Jul. 2010), page 1294-1304.
Topik: ß-catenin; glycogen synthase kinase (GSK)-3ß; celecoxib; MG-63 cell line; osteosarcoma; RNA interference (RNAI)

Ketersediaan

Perpustakaan FK Nomor Panggil: J11.K.2010.02 Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada

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Isi artikel

Cyclo-oxygenase (COX)-2 inhibitors may exert antitumour effects through COX-2-independent mechanisms. This study investigated the effects of the COX-2 inhibitor celecoxib on the viability of the human osteosarcoma MG-63 cell line and its beta-catenin signalling pathway. Cell viability and apoptosis were examined in celecoxib-treated cells or after beta-catenin knockdown in vitro. Analyses were performed to detect glycogen synthase kinase (GSK)-3b, phosphorylated GSK-3beta, beta-catenin, c-Myc and cyclin D1 proteins, and mRNA levels of beta-catenin, c-Myc and CCND1 (encoding cyclin D1). Beta-catenin was shown to be required for MG63 cell survival and celecoxib exerted an inhibitory effect on the viability of cultured MG-63 cells in a time- and dose-dependent manner. Beta-catenin protein decreased in the cytosol and nucleus following celecoxib treatment (from 6 h after initiation of treatment onwards; lowest protein levels were reached at > 72 h). Significant reductions in beta-catenin, c-Myc and CCND1 mRNA were observed. Celecoxib inhibited MG-63 cell viability, possibly by activating GSK-3beta and inhibiting beta-catenin-dependent gene transcription, suggesting a role for celecoxib in osteosarcoma treatment.

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