Detail High-resolution Mapping of Linear Antibody Epitopes Using Ultrahigh-density Peptide Microarrays (from Molecular & Cellular Proteomics 2012, 11, 1790-1800) Bibliografi Author: Buus, Soren ; Rockberg, Johan ; Forsstrom, Bjorn ; Nilsson, Peter ; Uhlen, Mathias ; Schafer-Nielsen, Claus Topik: Linear antibody epitopes - Mapping; Peptide microarrays; Seminar - Thesis lit Bahasa: (EN )     Penerbit: The American Society for Biochemistry and Molecular Biology (ASBMB)     Tempat Terbit: Maryland    Tahun Terbit: 2012     Jenis: Article - diterbitkan di jurnal ilmiah internasional Fulltext: zjw1790.pdf (2.78MB; 0 download) [Informasi yang berkaitan dengan koleksi ini di internet] Abstract Antibodies empower numerous important scientific, clinical, diagnostic, and industrial applications. Ideally, the epitope(s) targeted by an antibody should be identified and characterized, thereby establishing antibody reactivity, highlighting possible cross-reactivities, and perhaps even warning against unwanted (e.g. autoimmune) reactivities. Antibodies target proteins as either conformational or linear epitopes. The latter are typically probed with peptides, but the cost of peptide screening programs tends to prohibit comprehensive specificity analysis. To perform high-throughput, high-resolution mapping of linear antibody epitopes, we have used ultrahigh-density peptide microarrays generating several hundred thousand different peptides per array. Using exhaustive length and substitution analysis, we have successfully examined the specificity of a panel of polyclonal antibodies raised against linear epitopes of the human proteome and obtained very detailed descriptions of the involved specificities. The epitopes identified ranged from 4 to 12 amino acids in size. In general, the antibodies were of exquisite specificity, frequently disallowing even single conservative substitutions. In several cases, multiple distinct epitopes could be identified for the same target protein, suggesting an efficient approach to the generation of paired antibodies. Two alternative epitope mapping approaches identified similar, although not necessarily identical, epitopes. These results show that ultrahigh-density peptide microarrays can be used for linear epitope mapping. With an upper theoretical limit of 2,000,000 individual peptides per array, these peptide microarrays may even be used for a systematic validation of antibodies at the proteomic level. [seminar - thesis lit] Opini Anda Klik untuk menuliskan opini Anda tentang koleksi ini! Lihat Sejarah Pengadaan  Konversi Metadata   Kembali

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