| Biofilms are structured communities of microbial species attached in a biopolymer matrix on either biotic or abiotic surface. Biofilm acts as a protective barrier, enhances the bacteria’s nutrient preservation, and supports virulence mechanism of bacteria. As for infected individuals, the biofilm formation increases the severity of the disease because it became harder to resolve the disease using antibiotics. Therefore, many studies had been done in order to find antibiofilm compounds from many sources. The objective of this study is to explore the potential of MB 35 pigment as antibiofilm compound and characterize the stability of the pigment. From the previous study reported by Shanita (2012) and Deil (2015), marine bacteria MB 35 had been isolated from marine organisms (sea snails, anemones, and algae) and its pigment had been characterized and analyzed for their application as textile dyes. Based on this study, MB 35 pigment established biofilm inhibition and destruction activity against four biofilm-producing bacteria (S. aureus, EHEC, ETEC, and EPEC). The best inhibition activity was observed in 20 % of MB 35 pigment addition and the best destruction activity was observed in 5% of MB 35 pigment addition. The best MB 35 pigment stability was achieved at 25 °C, pH 4.34, and dark storage condition. The MB 35 pigment did not showed antibacterial activity against 12 test bacteria used in this study. |