Detail Purification of Biofilm inhibitor from marine bacteria (disajikan dalam Seminar Perhimpunan Biokimia dan Biologi Molekuler, Bali, Indonesia 2016) Bibliografi Author: Waturangi, Diana Elizabeth ; Chen, Casandra Bahasa: (EN )     Penerbit: Perhimpunan Biokimia dan Biologi Molekuler (PBBMI)     Tempat Terbit: Bali, Indonesia    Tahun Terbit: 2016     Jenis: Papers/Makalah - pada seminar nasional Fulltext: Purification of biofilm inhibitor.pdf (397.66KB; 12 download) Abstract Biofilm is an assemblage of microbial cells on biotic and abiotic surface embedded in polymeric matrix. Biofilm is formed as a mode of bacterial growth in adverse environment. Various antibiofilm compounds from different sources have been reported. These compounds are usually either polysaccharides, protein, enzymes, small molecules, or quorum sensing inhibitors. They can either come from plants extract, bacterial extract or even synthetic molecules. In this study actinomycetes from previous study were exhibited antibiofilm activity, we further study for CW01 isolate which were characterized as Arthrobacter sp. The bacterial crude extract were precipitated using etanol; dried and purified using Hi-Trap DEAE-Sepharose Fast Flow coloumn, the bioactive compound were then identified, and characterized for their antibiofilm activity. Purification result showed a single peak when eluted by linear gradient of NaCl. The concentration at which CW01 was eluted around 1.18 M NaCl. The antibiofilm activity of the crude and purified were tested against P. aerugionosa, S. aureus, S. pneumoniae, and ETEC. For inhibition assay, purified CW01 showed an increased in biofilm inhibition activity of P.aeruginosa and S. aureus. The increasing activity against P. aeruginosa was 4.3-fold for 5% sample CW01 concentration and 4.7-fold for 10%, whereas for S. aureus the increasing was 1.3-fold and 1.9-fold for 5% and 10% CW01 added respectively. There is no significant difference in biofilm inhibition activity of purified sample against S. pneumonia for 5% sample added but decreased for 10% CW01 added. Purified CW01 worked effectively to inhibit P. aeruginosa when compared with other pathogens. For biofilm destruction activity, purified CW01 only showed an increased in activity against P.aeruginosa. Biofilm destruction against P.aeruginosa was increased by 1.9-fold and 2-fold for 5% and 10% CW01 added respectively. Purified CW01 showed a decrease in activity for both 5% and 10% of sample added against S.aureus whereas there was no significant change in activity against S. penumoniae. CW01 was more effective against P. aeruginosa compared with other pathogens. In order to determine the molecule that contributes to the antiobiofilm activity of the samples, the purified samples were given enzymatic treatment using nuclease (Dnase I and Rnase A) and proteinase K. Antibiofilm activity of CW01 were decreased to 29-35% when subjected to proteinase K. This result showed that protein played an important role in antibiofilm activity. Further characterization of the bioactive compound were needed as well as the production. Finding of new metabolite with antibiofilm activity is important as alternative approach to treat pathogenic bacteria. Opini Anda Klik untuk menuliskan opini Anda tentang koleksi ini! Lihat Sejarah Pengadaan  Konversi Metadata   Kembali

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