Detail Expression, immunogenicity and diagnostic value of envelope proteins from an Egyptian hepatitis C virus isolate (from Arch Virol 2015, 160, 945-958) Bibliografi Author: Shawky, Heba ; Maghraby, Amany S. ; Solliman, Mohei El-Din ; El-Mokadem, Mehreshan T. ; Sherif, Mohamed M. ; Arafa, Azza ; Bahgat, Magmoud M. Topik: HCV; Egyptian; Isolate; Seminar - Thesis lit Bahasa: (EN )     Penerbit: Springer-Verlag     Tahun Terbit: 2015     Jenis: Article - diterbitkan di jurnal ilmiah internasional Fulltext: art_3A10.1007_2Fs00705-015-2334-1.pdf (5.66MB; 0 download) Abstract The present work aimed at 1) characterization of the E1 and E2 proteins (HCV-E) from an Egyptian hepatitis C virus genotype 4a (HCV-4a) isolate at the molecular and immunological level, 2) in silico identification of the B- and T-cell epitopes responsible for the immunogenicity of HCV-E, and 3) evaluation of the diagnostic potential of both the recombinant HCV-E and antibodies raised using mammalian expression constructs encoding the protein. The region encoding the E1 and E2 proteins was amplified by RT-PCR from RNA isolated from blood of a human infected with HCV-4 and cloned into the pSC-TA plasmid, and the sequence was verified and used to construct a neighbor-joining phylogenetic tree. The translated nucleotide sequence was used to predict the HCV-E secondary structure using the PREDICT-PROTEIN server and PSI-PRED. A 3D model of HCV-E was generated using the online tool 3Dpro. B- and T-cell epitopes were predicted using the online tools BCPred and Epijen v1.0, respectively. The HCV-E-encoding... [seminar - thesis lit] Opini Anda Klik untuk menuliskan opini Anda tentang koleksi ini! Lihat Sejarah Pengadaan  Konversi Metadata   Kembali

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