Detail Analysis of a Successful HCV-Specific CD8+ T Cell Response in Patients with Recurrent HCV-Infection After Orthotopic Liver Transplantation (from Liver Transplantation 2004, Vol. 10 (12), 1487-1496) Bibliografi Author: Gruener, Nobert Hubert ; Jung, Maria-Christina ; Ulsenheimer, Axel ; Gerlach, Joern Tilman ; Zachoval, Reinhart ; Diepolder, Helmut Michael ; Baretton, Gustavo ; Schauer, Rolf ; Pape, Gerd Rudolf ; Schirren, Carl Albrecht Topik: HCV; Orthotopic liver transplantation; Validation ref - 6 Bahasa: (EN )     Penerbit: American Association for the Study of Liver Disease     Tahun Terbit: 2004     Jenis: Article - diterbitkan di jurnal ilmiah internasional Fulltext: 20300_ftp.pdf (157.93KB; 0 download) Abstract Virus-specific CD8 T cells play a major role in antiviral immune defenses; their significance in the transplant setting, however, is unclear. In the present study, we asked whether hepatitis C virus (HCV)-specific CD8 T cells were detectable in the presence of an immunosuppressive treatment and whether the HCV-specific CD8 T cell response correlates with treatment outcome in patients who receive interferon (IFN)- / ribavirin therapy after orthotopic liver transplantation (OLTx). Liver- and blood-derived T cell lines of 21 patients after OLTx were studied before, at the end of, and after antiviral treatment. Virus-specific IFN- production in response to a panel of previously identified HCV-specific epitopes restricted by the human leukocyte antigen (HLA) class I molecules A2, A3, B7, B35, and B44 of structural and nonstructural HCV protein was determined by enzyme-linked immunospot (ELISPOT) assay. Before treatment, only low numbers of HCV-specific CD8 T cells were detectable. In 6 patients with a sustained virological response, a significant, multispecific, and sustained CD8 T cell response was detectable, which was mainly found in the peripheral blood. Nonresponders and transient responders showed undetectable, weak, or transient HCV-specific CD8 T cell responses. (Sustained responders vs. transient and nonresponders: Wilcoxon rank-signed test; P < .01). In conclusion, our data indicate that despite immunosuppression, HCV-specific CD8 T cells are detectable in patients with recurrent HCV infection after OLTx and that a significant, multispecific, and long-lasting HCVspecific CD8 T cell response contributes to viral elimination. [validation ref - 6] Opini Anda Klik untuk menuliskan opini Anda tentang koleksi ini! Lihat Sejarah Pengadaan  Konversi Metadata   Kembali

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