Detail Sequence uniqueness and sequence variability as modulating factors of human anti-HCV humoral immune response (from Cancer Immunol Immunother (2008), 57, 1215-1223) Bibliografi Author: Kanduc, Darja ; Tessitore, Luciana ; Lucchese, Guglielmo ; Kusalik, Anthony ; Farber, Emanuel ; Marincola, Francesci M. Topik: Unique peptide sequences; Conserved peptide sequences; Sequence-to-sequence peptide matching; Proteasomal cleavages; HCV-related immunity; Validation ref - 9 Bahasa: (EN )     Penerbit: Springer-Verlag     Tahun Terbit: 2008     Jenis: Article - diterbitkan di jurnal ilmiah internasional Fulltext: art_3A10.1007_2Fs00262-008-0456-y.pdf (446.47KB; 0 download) Abstract We recently compared the HCV polyprotein to the human proteome in order to test whether amino acid sequences unique to the virus could represent immunodominant epitopic determinants of the human humoral immune response against HCV. We identiWed a relatively limited number of HCV fragments with no/low similarity to the human host that represented exclusive HCV motifs. In this study, the peptides corresponding to low/zero similarity sequences were synthesized and assayed with HCVinfected sera. With diVerent patterns, the synthetic HCV peptides corresponding to low/zero similarity sequences were found to be immunoreactive. In particular, the HCV E1 (315–323) HRMAWDMMM, HCV E2/NS1 (547–555) NWFGCTWMN, and HCV NS5 (2638–2646) YDTRC FDST sequences were immunodominant in the HCVinfected cohort under study. These three peptides correspond to sequences that are endowed with low-similarity to the human proteome, are highly conserved among various HCV strains, and have, potentially, a scarce susceptibility to proteolytic attacks. These data may be of help in defining the multiple factors which concur in the modulation of the human immune response against HCV, eventually providing information for the design of eVective anti-HCV vaccines. [validation ref - 9] Opini Anda Klik untuk menuliskan opini Anda tentang koleksi ini! Lihat Sejarah Pengadaan  Konversi Metadata   Kembali

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