Reduced percentage of natural killer cells associated with impaired cytokine network in the secretory endometrium of infertile women with polycystic ovary syndrome  

Oleh:

Matteo, Maria ; Serviddio, Gaetano ; Massenzio, Francesca

Jenis: Article from Journal - ilmiah internasional
Dalam koleksi: Fertility and Sterility (keterangan: ada di ClinicalKey) vol. 94 no. 06 (Nov. 2010), page 2222-2227.
Topik: Infertility; uterine natural killer cells; PCOS; secretory endometrium; IL-15; IL-18; CXCL10

Ketersediaan

Perpustakaan FK Nomor Panggil: F02.K.2010.06 Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada

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Isi artikel

Objective To evaluate lymphocyte subset distribution in the secretory endometrium from infertile patients with polycystic ovary syndrome (PCOS), and the expression of the cytokines known to play a role in determining the endometrial lymphocyte pattern. Design Experimental clinical study. Setting Outpatient clinic in a university hospital. Patient(s) Twenty-eight patients with PCOS (PCOS group) and 6 fertile patients (control group). Intervention(s) On days 22–26 of a spontaneous cycle, subjects underwent endometrial biopsies. Main Outcomes Measure(s) In 19 of 28 patients with PCOS and 6 controls with a late secretory endometrium, the percentage and phenotype of lymphocyte subsets were analyzed by flow cytometry. In the late secretory endometrium of 11 patients with PCOS and 3 controls, the expression of interleukins 15 and 18 and of chemokine ligand 10 was also analysed by polymerase chain reaction. Result(s) In patients with PCOS the percentage of CD56+/CD16- and of CD56bright/CD16- cells was significantly lower (median [confidence interval]: 38% [31%–52.7%] vs. 63.7% [57.7%–69%] and 17.4% [8%–41.6%] vs. 52% [43%–60%], respectively), whereas the percentage of CD3+ was significantly higher (45% [33.3%–64%] vs. 26.1% [21%–32%]) as compared with controls. Accordingly, polymerase chain reaction analysis revealed a significantly lower expression of interleukins 15 and 18 and of chemokine ligand 10 in patients with PCOS than in controls. Conclusion(s) Results demonstrated an abnormal percentage of endometrial lymphocyte subsets, associated with an impaired cytokine network in patients with PCOS. This could explain the poor reproductive potential in these patients.

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