Pengembangbiakan Bacillus thuringiensis H-14 Galur Lokal Pada Berbagai Macam pH Media Air Kelapa dan Toksitisitasnya Terhadap Jentik Nyamuk Vektor Aedes aegypti dan Anopheles aconitus  

Oleh:

Susanti, Lulus ; Ch.P, Blondine

Jenis: Article from Journal - ilmiah nasional - terakreditasi DIKTI
Dalam koleksi: Media Penelitian dan Pengembangan Kesehatan vol. 20 no. 01 (Mar. 2010), page 9-16.
Topik: B. thuringiensis H-14; pH; Coconut Water Media
Fulltext: M45 Vol 20, No 1 Mar (2010) p9.PDF (539.2KB)

Ketersediaan

Perpustakaan PKPM Nomor Panggil: M45 Non-tandon: tidak ada Tandon: 1	Lihat Detail Induk
Perpustakaan FK Nomor Panggil: M32.K.2 Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada	Lihat Detail Induk

Isi artikel

The culture of bioinsecticide containing active Bacillus thuringiensis H-14 local strain on various kinds on coconut water pH and its toxicity against Aedes aegypti and Anopheles aconitus were carried out in the laboratory on Institute of Vector and Reservoir Control Research and Development Salatiga. The objectives of this study were: To determine the optimum pH from various kinds of coconut water pH for culturing of B. thuringiensis H-14 local strain. This study was using 2,0 coconuts with 6 - 8 months age coconut on average weight around 1 kg that contained water approximately 400-500 mll coconut were taken from Kunir Rejo village, Butuh regency, Purworejo district. Fifteen out of 20 coconuts were used to culture cells and spores of B. thuringiensis H-14 local strain and 5 coconuts were used to analyze the contain of coconut water in the Institute of Health Laboratory Semarang. The results showed,thatB. thuringiensis H-14 local strain can culture at ranges from pH 7 topH 8.5 with the pH7 as theoptimum pH Total Viable Cell (TVC) and a Total Viable Spore Count (TVSC) were 3,5 x 1010 cells/ml and 3, 3 x 1010 spores/ml respectively. The Lethal Concentration (LC 50) = 10.56 ppm and LC 95= 22. 13 ppm against Ae. agypti larvae and LC50 = 5 ppm and LC95 = 11 ppm against An. a conitus larvae. The result showed the analyze test contain of coconut water were 1.92 % carbohydrate,0.01 %fat, 0.06 % protein and reduced glucose 1.87 %. Coconut water can be used as an alternative local media to culture B. thuringiensis H-14 local strain.

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