Pengaruh Pemberian Elisitor Ekstrak Ragi (Saccharomyces Cerevisiae Hansen) Terhadap Kandungan Ajmalisin pada Kultur Kalus Catharantus Roseus (L.) G. Don  

Oleh:

Silalahi, Marina

Jenis: Article from Journal - ilmiah nasional - tidak terakreditasi DIKTI
Dalam koleksi: SIGMA: Jurnal Sains dan teknologi vol. 13 no. 1 (Jan. 2010), page 9-15.
Topik: Ajmalicine; Elicitor; HPLC; C. Roseus; Callus

Ketersediaan

Perpustakaan Pusat (Semanggi) Nomor Panggil: SS25 Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada

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Isi artikel

An experiment to study the effect of elicitor derived from Saccharomyces cerevisiae (Hansen) on ajmalicine content of Catharanthus roseus (L.) G. Don. callus cultures has been conducted. Callus was induced from leaf segment and grew on medium Zenk (1977) supplemented with 2,5 x 10"6 M NAA and 10"5 M BAP. Callus on the third subculture level was elicited with elicitor derived from S. cerevisiae. The following concentrations of elicitor tasted were 0 ; 0,5 ; 1,0 ; and 2,5% (g/v), and the harvesting times were 0, 18, 36 and 72 hour. The ajmalicine was analized qualitatively and quantitatively by using high performance liquid chromatography (HPLC). Ajmalicine content was influenced by concentration of elicitor and harvesting time. A significant increase of ajmalicine content (303,475 ± 5,602 µg/gDW) was achieved by addition of elicitor of 0,5% (g/v) after 36 hour. This study show a significant increase of ajmalicine content in C. roseus callus cultures after being challenged with S. cerevisiae elicitor i.e. 69,334%.

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