Dynamics of cohesin proteins REC8, STAG3, SMC1ß and SMC3 are consistent with a role in sister chromatid cohesion during meiosis in human oocytes  

Oleh:

Garcia-Cruz, R. ; Brieno, M.A. ; Roig, I.

Jenis: Article from Journal - ilmiah internasional
Dalam koleksi: Human Reproduction vol. 25 no. 09 (Sep. 2010), page 2316-2327.
Topik: * sister chromatid cohesion * cohesin * meiosis * oocyte * chromosome non-disjunction

Ketersediaan

Perpustakaan FK Nomor Panggil: H07.K.2010.03 Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada

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BACKGROUND Sister chromatid cohesion is essential for ordered chromosome segregation at mitosis and meiosis. This is carried out by cohesin complexes, comprising four proteins, which seem to form a ring-like complex. Data from animal models suggest that loss of sister chromatid cohesion may be involved in age-related non-disjunction in human oocytes. Here, we describe the distribution of cohesins throughout meiosis in human oocytes. METHODS We used immunofluorescence in human oocytes at different meiotic stages to detect cohesin subunits REC8, STAG3, SMC1ß and SMC3, [also synaptonemal complex (SC) protein 3 and shugoshin 1]. Samples from euploid fetuses and adult women were collected, and 51 metaphase I (MI) and 113 metaphase II (MII) oocytes analyzed. SMC1ß transcript levels were quantified in 85 maturing germinal vesicle (GV) oocytes from 34 women aged 19–43 years by real-time PCR. RESULTS At prophase I, cohesin subunits REC8, STAG3, SMC1ß and SMC3 overlapped with the lateral element of the SC. Short cohesin fibers are observed in the oocyte nucleus during dictyate arrest. All four subunits are observed at centromeres and along chromosomal arms, except at chiasmata, at MI and are present at centromeric domains from anaphase I to MII. SMC1ß transcripts were detected (with high inter-sample variability) in GV oocytes but no correlation between SMC1ß mRNA levels and age was found. CONCLUSIONS The dynamics of cohesins REC8, STAG3, SMC1ß and SMC3 suggest their participation in sister chromatid cohesion throughout the whole meiotic process in human oocytes. Our data do not support the view that decreased levels of SMC1ß gene expression in older women are involved in age-related non-disjunction.

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