Effect of dienogest administration on angiogenesis and hemodynamics in a rat endometrial autograft model  

Oleh:

Katayama, Hiroko ; Katayama, Tomihiro ; Uematsu, Kazuhiko

Jenis: Article from Journal - ilmiah internasional
Dalam koleksi: Human Reproduction vol. 25 no. 11 (Nov. 2010), page 2851-2858.
Topik: dienogest; endometriosis; angiogenesis; dorsal skinfold chamber; intravital fluorescence microscopy

Ketersediaan

Perpustakaan FK Nomor Panggil: H07.K.2010.04 Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada

Lihat Detail Induk

Isi artikel

BACKGROUND We aimed to establish an endometrial autograft model in rats that would allow for repetitive in vivo analyses of angiogenesis. Dienogest (DNG) is an orally active progestin used for the treatment of endometriosis. We investigated whether DNG would affect angiogenesis of the ectopic endometrium in our model. METHODS Mechanically isolated endometrial fragments were transplanted into dorsal skinfold chambers in rats. We analyzed the effect of DNG on angiogenesis of the ectopic endometrium on Days 0, 2, 4, 7, 10 and 14 after transplantation using intravital fluorescence microscopy. RESULTS The DNG-administered group showed significant suppression of angiogenesis of endometrial autografts, as indicated by the reduced size of the microvascular network and decreased microvessel density compared with those of control animals. The newly formed microvessels of the DNG-administered group showed consistently elevated diameters and centerline red blood cell velocity was decreased. Immunohistochemistry revealed a significant reduction in the level of perivascular a-smooth muscle actin within endometrial grafts of the DNG-administered group. CONCLUSIONS DNG inhibited angiogenesis of the ectopic endometrium, with confirmed structural changes in the microvessels.

Opini Anda

Klik untuk menuliskan opini Anda tentang koleksi ini!

Kembali