Background: Lymphatic filariasis (LF) is a tropical disease which is transmitted by mosquito bites, one of them is Culex sp. Almost 1.4 billion people around the world are at risk of LF. One attempt to eliminate LF is performing Integrated Vector Control by using liquid vaporizer. Peppermint oil was extracted from mint leaves (Mentha piperita L.) as an environment safety-insecticide against Culex sp. Methods: The design of this research is the true experimental-post test only control group. The number of mosquitoes used for each experiment was 10. The testing concentrations of peppermint oil were 0,2%; 0,4%; 0,8%; 1,6%; and 3,2% which was fumigated and the lethal effect was observed at 0,5 hour, 1 hour, 2 hours, 4 hours, and 6 hours. Result: There is a significant difference of the lethal effect between the mosquitoes before and after fumigation which p = 0,041 (p < 0,05). The concentration of peppermint oil which resulted in 100% lethal effect was 3,2%. Conclusion: The peppermint oil in five concentrations; 0,2%; 0,4%; 0,8%; 1,6%; and 3,2% have the lethal effect as an insecticide against the Culex sp. The higher the concentration of the peppermint oil, the largest number of Culex sp. got the lethal effect. |