Comparison of Radioimmunoprecipitation With Luciferase Immunoprecipitation for Autoantibodies to GAD65 and IA-2ß  

Oleh:

Burbelo, Peter D. ; Hirai, Hiroki ; Issa, Alexandra T.

Jenis: Article from Journal - ilmiah internasional
Dalam koleksi: Diabetes Care vol. 33 no. 04 (Apr. 2010), page 754-756.
Topik: Radioimmunoprecipitation

Ketersediaan

Perpustakaan FK Nomor Panggil: D05.K.2010.02 Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada

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Isi artikel

OBJECTIVE To compare the sensitivity and specificity of luciferase immunoprecipitation (LIPS) with radioimmunoprecipitation (RIP) for the measurement of autoantibodies to the type 1 diabetes autoantigens glutamic acid decarboxylase 65 (GAD65) and insulinoma-associated protein (IA)-2ß. RESEARCH DESIGN AND METHODS Sera from 49 type 1 diabetic patients and 100 nondiabetic control subjects from Diabetes Antibody Standardization Program 2007 were used to screen for autoantibodies to GAD65. An additional 200 type 1 diabetic patients and 200 nondiabetic control subjects were used to validate the GAD65 results and screen for autoantibodies to IA-2ß. RESULTS LIPS showed equal sensitivity and specificity to RIP for detecting autoantibodies to GAD65 and IA-2ß. Receiver-operating characteristic analysis revealed that the detection of autoantibodies to GAD65 and IA-2ß by LIPS and RIP were not statistically different. CONCLUSIONS The LIPS assay does not require the use of radioisotopes or in vitro transcription/translation and is a practical alternative at the clinical level for the RIP assay.

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