Cell plating density alters the ratio of estrogenic to progestagenic enzyme gene expression in cultured granulosa cells  

Oleh:

Portela, Valerio M. ; Zamberlam, Gustavo ; Price, Christopher A.

Jenis: Article from Journal - ilmiah internasional
Dalam koleksi: Fertility and Sterility (keterangan: ada di ClinicalKey) vol. 93 no. 06 (Apr. 2010), page 2050-2055.
Topik: Cell number; granulosa; culture; serum-free; steroidogenesis; FACS; aromatase

Ketersediaan

Perpustakaan FK Nomor Panggil: F02.K.2010.02 Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada

Lihat Detail Induk

Isi artikel

Objective To determine if initial cell plating density alters steroidogenesis and the E2:P ratio in granulosa cells in long-term serum-free culture. Design Experimental study. Setting Academic institution. Animal(s) Cattle of slaughterhouse origin. Intervention(s) Culture of granulosa cells in vitro at different cell plating density. Main Outcome Measure(s) Steroid secretion was measured by RIA, mRNA levels were measured by real-time polymerase chain reaction, and cell death was assessed by flow cytometry. Result(s) Low plating density favored E2 secretion and mRNA encoding estrogenic enzymes, whereas higher density inhibited E2 secretion and enhanced P secretion and levels of mRNA encoding progestagenic enzymes. Increasing plating density decreased the E2:P ratio and cell health. Conclusion(s) Lower cell density favors an estrogenic granulosa cell phenotype, whereas higher density favors luteinization. Serum-free culture systems should be optimized with this in mind.

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