Effect of varying equilibration time in a two-step vitrification method on the post-warming DNA integrity of mouse blastocysts  

Oleh:

Kader, Amr ; Choi, Audrey ; Sharma, Rakesh K.

Jenis: Article from Journal - ilmiah internasional
Dalam koleksi: Fertility and Sterility (keterangan: ada di ClinicalKey) vol. 93 no. 08 (Jun. 2010), page 2640-2645.
Topik: Blastocyst; vitrification; cryopreservation; media protocol; TUNEL; apoptotic index; DNA integrity index

Ketersediaan

Perpustakaan FK Nomor Panggil: F02.K.2010.03 Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada

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Isi artikel

Objective To assess the effect of equilibration time on the DNA integrity of vitrified-warmed mouse blastocysts. Design Prospective in vitro study. Setting Embryology research laboratory. Intervention(s) Mouse nonexpanded blastocysts (NEB) (n = 54) and expanded blastocysts (EB) (n = 56) were vitrified using cryotips. Blastocysts were vitrified using a two-step media protocol, with a first (equilibration) step of 4 minutes [NEB (n = 20), EB (n = 24)], 8 minutes [NEB (n = 17), EB (n = 16)], or 15 minutes: [NEB (n = 17), EB (n = 16)]. Control samples were fresh NEB (n = 17) and EB (n = 18) blastocysts. Main Outcome Measure(s) DNA integrity index (DII) using terminal deoxynucleotide transferase–mediated dUTP nick-end labeling, confocal imaging, and viability. Result(s) 1) The DII of the vitrified warmed NEB and EB improved significantly with 8-minute equilibration protocol compared with 4 minutes. 2) The DII of the EB significantly decreased with 15-minute equilibration protocol compared with 4 or 8 minutes. 3) The DII in the NEB significantly improved with 8- and 15-minute equilibration protocols compared with 4 minutes, with no significant difference between 8 or 15 minutes. Conclusion(s) Vitrification with an 8-minute equilibration step improved DII of the NEB and EB after warming

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