Effect of vitrification and beta-mercaptoethanol on reactive oxygen species activity and in vitro development of oocytes vitrified before or after in vitro fertilization  

Oleh:

Gupta, Mukesh Kumar ; Sang Jun, Uhm ; Hoon Taek, Lee

Jenis: Article from Journal - ilmiah internasional
Dalam koleksi: Fertility and Sterility (keterangan: ada di ClinicalKey) vol. 93 no. 08 (Jun. 2010), page 2602-2607.
Topik: Oocyte vitrification; ROS activity; cryopreservation; fertility preservation; porcine

Ketersediaan

Perpustakaan FK Nomor Panggil: F02.K.2010.03 Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada

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Isi artikel

Objective To investigate the effect of vitrification and beta-mercaptoethanol (ß-ME) on reactive oxygen species (ROS) activity and in vitro development of oocytes vitrified before or after in vitro fertilization (IVF). Design Randomized prospective study. Setting University-based assisted reproductive technology laboratory. Animals(s) Abattoir-derived porcine ovaries. Interventions(s) Oocytes were vitrified either before or 4 hours after the end of IVF by solid surface vitrification (SSV) without centrifugation and/or delipation procedure. ß-ME was used to inhibit ROS activity. Main Outcome Measures(s) Viability was evaluated by membrane integrity and esterase enzyme activity using fluorescein diacetate staining while ROS activity was assessed by 2',7'-dichlorofluorescein assay. Result(s) Vitrification increased the ROS activity and decreased the viability and in vitro development of vitrified oocytes. Addition of ß-ME to vitrification and culture medium partially annihilated the ROS activity but did not improve the viability of vitrified-warmed oocytes. Furthermore, ß-ME had no effect on improving the fertilization ability of oocytes vitrified at metaphase II stage but significantly increased their ability to cleave. ß-ME also increased the rate of cleavage and blastocyst formation ability of oocytes vitrified 4 hours after the end IVF. Conclusion(s) Vitrification increases ROS activity in oocytes that can be partially annihilated by ß-ME to obtain enhanced embryonic development.

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