Transgenerational changes in somatic and germ line genetic integrity of first-generation offspring derived from the DNA damaged sperm  

Oleh:

Adiga, Satish Kumar ; Upadhya, Dinesh ; Kalthur, Guruprasad

Jenis: Article from Journal - ilmiah internasional
Dalam koleksi: Fertility and Sterility (keterangan: ada di ClinicalKey) vol. 93 no. 08 (Jun. 2010), page 2486-2490.
Topik: Sperm DNA damage; preimplantation embryos; genomic instability; transgenerational effect

Ketersediaan

Perpustakaan FK Nomor Panggil: F02.K.2010.03 Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada

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Isi artikel

Objective To report a more quantitative approach to study the influence of varying levels of sperm DNA damage on transgenerational changes in genomic instability in a mouse model. Design Experimental prospective study. Setting Embryology research laboratory. Animal(s) Swiss albino mice. Intervention(s) The sperm DNA damage was induced by different doses of ?-irradiation to male mice followed by mating with healthy female mice. Main Outcome Measure(s) Genomic integrity in embryos, fetus, and spermatozoa of F1 mice derived from the DNA-damaged sperm. Result(s) The transgenerational changes in genetic integrity were attributed by a dose-dependent increase in the frequency of micronuclei in preimplantation embryos and a concomitant increase in genomic instability in fetal liver cells and sperm chromatin modifications in F1 males. A strong positive correlation was observed between the extent of sperm DNA damage and somatic and germ-line genomic instability. Conclusion(s) Sperm-mediated transgenerational genomic instability is dependent on the amount of DNA damage present in the sire's sperm at the time of fertilization.

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