Alterations in sperm DNA methylation patterns at imprinted loci in two classes of infertility  

Oleh:

Hammoud, Saher Sue ; Purwar, Jahnvi ; Pflueger, Christian

Jenis: Article from Journal - ilmiah internasional
Dalam koleksi: Fertility and Sterility (keterangan: ada di ClinicalKey) vol. 94 no. 05 (Oct. 2010), page 1728-1733.
Topik: Imprinting; Beckwith-Wiedemann syndrome and epigenetic alterations; Angelman syndrome; chromatin; assisted reproductive technology; IVF; ICSI; oligozoospermic; protamines

Ketersediaan

Perpustakaan FK Nomor Panggil: F02.K.2010.05 Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada

Lihat Detail Induk

Isi artikel

Objective To evaluate the associations between proper protamine incorporation and DNA methylation at imprinted loci. Design Experimental research study. Setting Research laboratory. Patient(s) Three populations were tested—abnormal protamine patients, oligozoospermic patients, and fertile donors. Intervention(s) The CpG methylation patterns were examined at seven imprinted loci sequenced: LIT1, MEST, SNRPN, PLAGL1, PEG3, H19, and IGF2. Main Outcome Measure(s) The DNA methylation patterns were analyzed using bisulfite sequencing. The percentage of methylation was compared between fertile and infertile patients displaying abnormal protamination. Result(s) At six of the seven imprinted genes, the overall DNA methylation patterns at their respective differentially methylated regions were significantly altered in both infertile patient populations. When comparing the severity of methylation alterations among infertile patients, the oligozoospermic patients were significantly affected at mesoderm-specific transcript (MEST), whereas abnormal protamine patients were affected at KCNQ1, overlapping transcript 1 (LIT1), and at small nuclear ribonucleoprotein polypeptide N (SNRPN). Conclusion(s) Patients with male factor infertility had significantly increased methylation alteration at six of seven imprinted loci tested, with differences in significance observed between oligozoospermic and abnormal protamine patients. This could suggest that risk of transmission of epigenetic alterations may be different with diagnoses. However, this study does not provide a causal link for epigenetic inheritance of imprinting diseases, but does show significant association between male factor infertility and alterations in sperm DNA methylation at imprinted loci.

Opini Anda

Klik untuk menuliskan opini Anda tentang koleksi ini!

Kembali