Ethinylestradiol and estradiol have different effects on oxidative stress and nitric oxide synthesis in human endothelial cell cultures  

Oleh:

Andozia, Mayra Beraldo ; Vieira, Carolina Sales ; Franceschini, Silvio Antonio

Jenis: Article from Journal - ilmiah internasional
Dalam koleksi: Fertility and Sterility (keterangan: ada di ClinicalKey) vol. 94 no. 05 (Oct. 2010), page 1578-1582.
Topik: Ethinylestradiol; nitric oxide; antioxidant; endothelial cells; cardiovascular disease

Ketersediaan

Perpustakaan FK Nomor Panggil: F02.K.2010.05 Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada

Lihat Detail Induk

Isi artikel

Study objective To compare the effects of ethinylestradiol (EE) and 17ß-estradiol (E2) on nitric oxide (NO) production and protection against oxidative stress in human endothelial cell cultures. Design Experimental study. Settings Research laboratory. Material Human ECV304 endothelial cell cultures. Intervention(s) The NO synthesis was determined by flow cytometry, and oxidative stress was determined by a cell viability assay, after exposure to hydrogen peroxide (H2O2) and stimulation of endothelial cells with EE at concentrations similar to those of a contraceptive containing 30 µg EE. Main Outcome Measure(s) The effects of EE were compared with those of E2 at concentrations similar to those occurring during the follicular phase. Result(s) Ethinylestradiol did not increase NO synthesis and did not protect cells against oxidative stress. The viability of the cells incubated with E2 in combination with H2O2 was greater than the viability obtained with H2O2 only or with H2O2 in combination with EE. The cells stimulated with E2 presented a significant increase in NO production compared with control. Conclusion(s) In contrast to the effects of E2, EE did not protect human ECV304 endothelial cells against oxidative stress and did not increase their production of NO.

Opini Anda

Klik untuk menuliskan opini Anda tentang koleksi ini!

Kembali