A novel single-cell DNA fingerprinting method successfully distinguishes sibling human embryos  

Oleh:

Treff, Nathan R. ; Jing, Su ; Xin, Tao

Jenis: Article from Journal - ilmiah internasional
Dalam koleksi: Fertility and Sterility (keterangan: ada di ClinicalKey) vol. 94 no. 02 (Jul. 2010), page 477-484.
Topik: Blastomere; DNA fingerprinting; DNA microarray; single nucleotide polymorphism; whole genome amplification

Ketersediaan

Perpustakaan FK Nomor Panggil: F02.K.2010.04 Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada

Lihat Detail Induk

Isi artikel

Objective To validate a novel system for embryonic DNA fingerprinting which can reliably distinguish sibling embryos from each other. Design Prospective, randomized, and blinded study. Setting Academic center for reproductive medicine. Patient(s) Blastomeres were obtained from discarded and transferred embryos from six patients undergoing IVF treatment. Intervention(s) None. Main Outcome Measure(s) Single lymphocytes from sibling cell lines and blastomeres from sibling day 3 human embryos were evaluated for accurate assignment of relationship using whole genome amplification and single-nucleotide polymorphism microarrays. Result(s) Assignment of single-cell relationships was accomplished with 100% accuracy. We also observed complete agreement between the molecular karyotype and DNA fingerprint–based identification of embryos implanted in three clinical IVF cases after multiple embryo transfer. Conclusion(s) These data demonstrate the first single-blastomere DNA fingerprinting technology capable of unequivocal discrimination of sibling human embryos.

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