Automatic user-independent zona pellucida imaging at the oocyte stage allows for the prediction of preimplantation development  

Oleh:

Ebner, Thomas ; Balaban, Basak ; Moser, Marianne

Jenis: Article from Journal - ilmiah internasional
Dalam koleksi: Fertility and Sterility (keterangan: ada di ClinicalKey) vol. 94 no. 03 (Aug. 2010), page 913-920.
Topik: Birefringence; inner layer; oocyte quality; polarization microscopy; zona pellucida; zona score

Ketersediaan

Perpustakaan FK Nomor Panggil: F02.K.2010.04 Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada

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Isi artikel

Objective To analyze whether a change in three-dimensional structure of the zona pellucida could indicate suboptimal gamete quality. Design Prospective study. Setting Women's general hospital. Patient(s) A total of 72 patients who gave informed consent. Intervention(s) The birefringence of all oocytes was prospectively analyzed with an automatic user-independent polarization microscopy imaging system. Main Outcome Measure(s) Birefringence of the inner zona layer, preimplantation development, implantation, and pregnancy. Result(s) In approximately one third of all gametes (244/712), the system's automatic detection of the inner zona layer did not succeed. This phenomenon was a negative predictor of compaction (P < 0.01), blastulation (P < 0.001), and pregnancy (P < 0.001). In cases of successful zona imaging, the score based on the birefringence of the inner zona layer was a strong predictor of blastocyst formation but not of embryo quality or pregnancy (P > 0.05). Interestingly, antagonist protocol resulted in lower zona scores as compared with the long protocol (P < 0.05). Conclusion(s) Combining the information from both undetected and detected oocytes, zona imaging was a helpful tool in oocyte selection. This knowledge might further help to reduce both the time in culture and the number of concepti considered for transfer.

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