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BukuTeknik Ligasi Duktus Koledokus Hati Untuk Model Kolestasis Ekstrahepatik Pada Tikus Sprague Dawley (dipresentasikan pada Pertemuan Ilmiah Nasional Perhimpunan Ahli Anatomi Indonesia (PAAI) Bandung, 24-26 Oktober 2013)
Bibliografi
Author: Harjanti, Dyonesia Ary ; Liem, Isabella K. ; Prijanti, Ani Retno ; Sidharta, Veronika Maria ; Bororing, Sheella Rima
Topik: Kolestasis ekstrahepatik; ligasi duktus koledokus; tikus Spraque dawley jantan; JABFUNG-FK-DAH-2015-03
Bahasa: (ID )    ISBN: 978-602-14509-0-1    
Penerbit: Fakultas Kedokteran Universitas Padjadjaran Bandung     Tempat Terbit: Bandung    Tahun Terbit: 2013    
Jenis: Papers/Makalah - pada seminar nasional
Fulltext:
Abstract
Background:
Extrahepatic cholestatic liver disease is a severe pathological condition that can lead to terminal liver disease. This condition can be caused by obstruction of the common bile duct amongst other causes. For the experimental study of extrahepatic cholestatic liver disease, an animal model is required. We therefore developed a choledochal duct ligation technique in Sprague-Dawley rats as a model of extrahepatic cholestasis.
Method:
The animal model used was a male Spraque Dawley male rat aged 8 weeks with an average body weight of 200-250 gram. Surgery was performed under general anesthesia using a mixture of xylazine 20 and ketamine 100 to a ratio of 1:1 at dose of 10 mg/kg body weight. The drug was administered to the rats by intramuscular injection (IM) at a dose of 0.1 ml in the thigh area and required approximately 15 minutes to achieve anaesthesia. A 4cm mid-sagital line incision was made in the umbilical area. Choledochal duct ligation was performed in two areas using monofilament 5/0 (Serafit). Wound closure was achieved with two types of stitching; to the subcutaneous layer with a single node technique using monofilament 5/0 (Serafit) and stitching the layers of the epidermis with a simple baste technique using monofilament 3/0 (Serafast). To prevent fatality due to anaesthesia overdose, it was reversed by injecting Yohimbine (Reverzine) 0.1 ml after surgery. Surgery was performed within 15 minutes.
Results:
One day post-operatively, the signs of biliary stasis appeared, with green tinged faeces and yellowing of the skin tail, ears and conjunctivae. Macroscopic histopathological examination of liver tissue showed signs of swollen liver tissue, dilated sinusoids and dilated proximal choledochal ducts with bile-green appearance. Microscopic examination using routine Hematoxilin-Eosin stain showed proliferation of liver bile ducts.
Conclusion:
Ligation of the choledochal duct in two regions may be used as a technique to reproduce a model of extrahepatic cholestasis.
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