PERANAN ISOZIM PROTEIN KINASE C-ALPHA DALAM MEMEDIASI EFEK ANTI PROLIFERASI ALPHA-TOKOFEROL PADA SEL STROMA PROSTAT  

Oleh:

Purnomo, Basuki B.

Jenis: Article from Journal - ilmiah nasional - terakreditasi DIKTI
Dalam koleksi: Jurnal Urologi Indonesia (Indonesian Journal of Urology) vol. 16 no. 02 (Jul. 2009), page 29-36.
Topik: Protein kinase c-alpha; primary culture; human prostatic stromal cells; antiproliferative effect

Ketersediaan

Perpustakaan FK Nomor Panggil: J33.K.01 Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada

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Isi artikel

Objective: Alpha tocopherol, also has a non anti-oxidant activity that correlates with inhibition of PKC-alpha isozyme activity. Several studies confirmed its role in inhibition of vascular smooth muscle proliferation. Inhibition of stromal cell proliferation is an alternative method of prevention of BPH. The aim of this in vitro study is to confirm that alpha-tocopherol, by its ability to inhibit PKC-alpha activity, has the capability to inhibit human prostatic stromal cell proliferation. Materials & Methods: Primary cultures of human prostatic stromal muscle cells were obtained from 4 patients with bladder outlet obstruction. The effects of various concentrations of alpha-tocopherol (20, 30, 40, 50, and 60 microM) were examined by cell proliferation and PKC-alpha isozyme activity assay. The cell proliferation study was performed using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay method. A caspase-3 activity assay was also performed to examine apoptotic mechanism. Results: The MTT method, which is used to count viable cells, showed that exposures to various doses of alpha-tocopherol resulted in a decreased proliferation rate. The decrement of proliferation rate is linear with the increment of alpha-tocopherol dose. Immunocytochemistry staining with anti caspase-3 antibody showed that no cells developed apoptosis. Alpha¬-tocopherol also inhibits PKC-alpha activity. Isozyme activity is reduced along with the increment of the dose (dose¬dependent). There is a correlation between PKC-alpha activity with cell proliferation rate after exposure to alpha-¬tocopherol (R2=0,9279; p=0, 000). Conclusion: Alpha-tocopherol at different concentrations reduces prostatic stromal cell proliferation, without an effect on apoptosis. This antiproliferative property correlates with a decreased activity of PKC-alpha isozyme.

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