EFEK PAPARAN GLUKOSA KONSENTRASI TTNGGi TERHADAP PENINGKATAN AKTIVITAS ISOZIM PROTEIN KINASE C (PKC)-ALPHA PADA KULTUR SEL STROMA PROSTAT  

Oleh:

Purnomo, Basuki B. ; Widharse, I Wayan

Jenis: Article from Journal - ilmiah nasional - terakreditasi DIKTI
Dalam koleksi: Jurnal Urologi Indonesia (Indonesian Journal of Urology) vol. 16 no. 01 (Jan. 2009), page 1-5.
Topik: Protein kinase. human prostatic stroma cell culture. high glucose. dynamic component

Ketersediaan

Perpustakaan FK Nomor Panggil: J33.K.01 Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada

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Isi artikel

Objective: To prove that glucose treatment in human prostatic stromal cells (HPSC) culture would increase PKC alpha izozyme activity: Materials and Methods: Priniary cultures of HPSC were obtained from patients with bladder outlet obstruction caused by benign prostatic enlargement. The effects of varions concentrations of glucose (5, 10, 15, 20, 25, 30, 35 micro-Mol) on PKC-alpha isozyme activity were examined quantitatively by spectrophotometry methods. Results: Spectrophotometry analysis. which is used to assess isozyme activity quantitatively showed that exposures to various doses of glucose resulted in increased isozyme activity. The increment of activity is linear with the increment of glucose dose. There is a strong. correlation between glucose doses and PKC alpha activity. with R2 =0,91. Conclusion: At different concentrations glucose increases PKC alpha isozyme activity of HPSC

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