EFEK 17P-ESTRADIOL TERHADAP DENSITAS RESEPTOR ADRENERGIK-aIO DAN KONTRAKTILITAS OTOT POLOS PEMBULUH DARAH TIKUS  

Oleh:

Nurdiana

Jenis: Article from Journal - ilmiah nasional
Dalam koleksi: Jurnal Kedokteran Brawijaya vol. 24 no. 02 (Aug. 2008), page 90-96.
Topik: 17ß-Estradiol; a10-adrenergic receptor; vascular smooth muscle contractility

Ketersediaan

Perpustakaan FK Nomor Panggil: J34.K.02 Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada

Lihat Detail Induk

Isi artikel

The incidence of hypertension in menopausal and post menopausal women related to plasma estrogen level and sympathetic nervous activity. Estrogen may have a specific effect to modulate adrenergic vasoconstriction by modulating adrenergic receptors. Blood vessels contractility is regulated mainly by the sympathetic nervous system, in aorta mediated particularly by a10 adrenergic receptor. We hypothesize that 17 ß estradiol decreases vascular smooth muscle contractility by decreasing a10 adrenergic receptor density. To prove the above mechanism in the present study we performed several interrelated assays, i.e bioassay using isolated organ and protein blotting. An isolated rat aortic ring without endothelium (2-3 month, 150-200 gram) was incubated in 17ß-estradiol (10-5 M, 10-4 M,and 10-3 M ) for two hours prior to stimulation using phenylephrine as a10 adrenergic receptor agonist. The response of the rat aortic ring to the stimulation of a10 adrenergic receptor agonist was measured as the subsequent change on aortic contractility using bioassay, and the change of a10 adrenergic receptor density using Western and dot blot. The result of this study showed that 17ß-estradiol decreases the rat aortic contraction significantly as a response to stimulation of phenylephrine (p=0,000). It is demonstrated as the higher level of 17 ß-estradiol, the more Emax of phenylephrine decreased without altering ED50 and pD2” also the amount density of a10 adrenergic receptor. To conclude, in the present study it is proved that 17ß-estradiol decreased aortic smooth muscle contractility which is associated with the decrease of the density of a10-adrenergic receptor.

Opini Anda

Klik untuk menuliskan opini Anda tentang koleksi ini!

Kembali