ANALISIS LOSS OF HETEROZYGOSITY GEN RAR B2 PADA PATOGENESIS KANKER PAYUDARA  

Oleh:

Muliartha, I Ketut Gede ; Riawan, Wibi ; Hidayati, Asnah ; Armania, Fitri

Jenis: Article from Journal - ilmiah nasional
Dalam koleksi: Jurnal Kedokteran Brawijaya vol. 24 no. 02 (Aug. 2008), page 86-89.
Topik: RAR ß2 gene; breast cancer; LOH

Ketersediaan

Perpustakaan FK Nomor Panggil: J34.K.02 Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada

Lihat Detail Induk

Isi artikel

The aim of this research was to investigate the event of loss of heterozygosity of retinoid acid receptor (RAR ß2) gene on the chromosom 3p24 in the pathogenesis of breast cancer. The breast cancer is the most malignant tumor that leads the second cause of death among the female. The recent concept of the theory of pathogenesis breast cancer is molecular genetic approaches such as apoptosis gene, oncogen, suppressor gene, and growth promoting factor gene. However, the diagnostic and therapy of breast cancer are still not satisfied. The lost or decreased in the expression of RAR ß2 gene on 3p24 chromosome via loss of heterozygosity (LOH) mechanism are the role of pathogenesis of breast cancer. Design of the research is by experimental and observational. Human female breast cancer and blood samples were gained from public hospital RSUD dr. Saiful Anwar, Malang. DNA was extracted from lymphocyte and tissue using Macherey-Nagel system kit. Loss of heterozygosity (LOH) incident in 3p24 chromosome was detected by PCR and Chromogenic in situ Hybridization (CISH) method. Result showed that LOH event has detected in chromosome 3p24 with the absence of several band of DNA by PCR. The LOH event also expressed in breast cancer tissues through the reduction of RAR ß2 expression as the grade of breast cancer increase. RAR ß2 gene is located at chromosome 3p24, and LOH event in chromosome 3p24 is common in breast cancer.

Opini Anda

Klik untuk menuliskan opini Anda tentang koleksi ini!

Kembali