EFEK KOMBINASI ARTEMISININ DAN N-acetylcysteine TERHADAP KADAR Malondialdehyde (MDA) OTAK DAN PARU MENCIT GALUR Balb/c YANG DIINFEKSI Plasmodium berghei  

Oleh:

Fitri, Loeki Enggar ; Iskandar, Agustin

Jenis: Article from Journal - ilmiah nasional
Dalam koleksi: Jurnal Kedokteran Brawijaya vol. 24 no. 02 (Aug. 2008), page 73-78.
Topik: Malaria; Artemisinin; N-acetylcysteine; MDA; Cerebral; Lung

Ketersediaan

Perpustakaan FK Nomor Panggil: J34.K.02 Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada

Lihat Detail Induk

Isi artikel

Cerebral and lung damage during malaria infection is believed to be caused by free radicals activities that are produced during immunology process. The free radicals react with lipid component of cellular membrane which generates malondialdehyde (MDA) as its end-product. The aim of the research was to determine whether combination of artemisinin and NAC was more effective in decreasing cerebral and lung MDA level compared to artemisinin mono-therapy. The research was post-test-control-only design using 5 groups consisted of group A (negative control group), group B mice which infected with P.berghei without therapy (positive control group), group C mice which infected with P.berghei and received artemisinin mono-therapy (0.04 mg/g BW for 7 days), group D mice which infected with P.berghei and received artemisinin in combination with NAC (1 mg/g BW for 7 days) and group E mice which infected with P.berghei and received artemisinin in combination with NAC (1 mg/g BW for 3 days and tapered into ½ mg/g BW for 4 days). On the 3rd, 5th, and 7th day, 3 mice from each group were scarified and assayed for MDA level. On the 3rd day, a decreasing trend of cerebral and lung MDA level was observed on all treatment groups. On the 5th day, a decreasing trend of cerebral and lung MDA level was observed in group that received artemisinin and NAC whereas group's that received artemisinin mono-therapy increased. Cerebral and lung MDA level of group that received artemisinin mono-therapy was significantly different with group that received combination of artemisinin and NAC in constant dose (p = 0.014) and with group that received combination artemisinin and NAC in tapering dose (p = 0.004). On the 7th day, cerebral MDA level of group that received combination artemisinin and NAC in constant dose was significantly lower than the group that received artemisinin mono-therapy (p = 0.005). In the lung, the MDA level of group that received artemisin mono-therapy increased, while the MDA level of combination group decreased. There was a significantly difference between group that receiving artemisinin mono-therapy and group that received combination of artemisinin and NA C tapering dose (p = 0.000). The conclusion is the combination of artemisinin and NAC is more effective in lowering cerebral and lung MDA level compared to artemisinin mono therapy on mice infected with P.berghei.

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