Detail Peranan mRNA Protein Disulfida Isomerase Family A, Member 4 (PDIA4) Sebagai Biomarker Baru untuk Deteksi Kejadian Metastasis Karsinoma Mammae Bibliografi Author: VANIARA, FLORENCIA VANYA ; Lembar, Stefanus (Advisor); Loanda, Evelyn (Advisor); Simon, Sumanto (Advisor) Topik: BREAST CANCER; PDIA4; Metastasis; Karsinoma Mammae; Breast Cancer Bahasa: (ID )     Penerbit: Fakultas Kedokteran Unika Atma Jaya     Tempat Terbit: Jakarta Utara    Tahun Terbit: 2014     Jenis: Theses - Karya Tulis Ilmiah Kedokteran (KTI-FK) Fulltext: Florencia_Vanya_Vaniara_KTI_2014.pdf (2.1MB; 1 download) Ketersediaan Perpustakaan FK Nomor Panggil: KTI-FK-663 Non-tandon: tidak ada Tandon: 1  Lihat Detail Induk Abstract Introduction Distant metastasis in breast cancer is attributable to a severe decrease in the five-year survival rate of patients from approximately 60% to 20%. Metastasis may be initiated by cellular hypoxia, which stimulates the migration of neoplastic cells towards distant sites. PDIA4 (Protein Disulphide-Isomerase family A, member 4) is thought to be a tumor biomarker of high sensitivity and specificity towards metastasized breast cancer. PDIA4 is a hypoxic stress protein that increases the cancerous cell’s resistance against hypoxia. The aim of this study is to find a correlation between the amount of PDIA4 within cancerous cells and the probability of distant metastasis in breast cancer for the purpose of finding a non-invasive and accurate means of diagnosing metastasis. Method mRNA samples from 21 metastasized and 18 non-metastasized breast cancer tissues were amplified for PDIA4 using reverse-transcription, qPCR and analyzed using Comparative CT Livak method. The Human ACTB (Beta-actin) gene was used as control. Results PDIA4 mRNA expression ratio is analyzed using the Livak method.Shapiro-Wilktest shows that the data distribution is abnormal (p<0,05). The mean value of PDIA4 mRNA expression ratio for the metastatic cell group is 4.72 and for the non-metastatic cell group is 3203.98. Based on Mann-Whitney correlation method, there is significance difference of mRNA PDIA4 expressions between the two groups (p<0.001). Result of Spearman correlation test is p=0.000 and R=-0.651 which mean there is significance strong correlation between mRNA PDIA4 expresssions towards metastasis status. Conclusion Metastatic breast cancer cells express a statistically significant lower level of PDIA4 mRNA when compared to non-metastatic breast cancer cells and influence the level of mRNA PDIA4. PDIA4 continues to be a promising biomarker for early detection of breast cancer metastasis. Opini Anda Klik untuk menuliskan opini Anda tentang koleksi ini! Lihat Sejarah Pengadaan  Konversi Metadata   Kembali

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