Detail Prevalence and and molecular characterization of Salmonella enteric Serovar Typhimurium from ice and beverages sold in Jakarta, Indonesia, using most probable number and multiplex PCR (presented at 15th International Congress of Infectious Diseases Bangkok, Thailand, 13-16 June 2012) Bibliografi Author: Waturangi, Diana Elizabeth ; Wiratama, Eko ; Theresia, Audora Topik: Salmonella enterica serovar Typhimurium; drinking water safety; ice and beverages; JABFUNG-DEW-2015-17 Bahasa: (EN )     Penerbit: International Society for Infectious Diseases     Tempat Terbit: Bangkok, Thailand    Tahun Terbit: 2012     Jenis: Poster Fulltext: B-17_Diana Waturangi.pdf (4.38MB; 6 download) Prevalence salmonella B17.pdf (115.25KB; 4 download) Abstract The presence of Salmonella enterica serovar Typhimurium is concern for the safety of drinking water and ice. Improper sanitation and poor hygiene can increase the salmonella infection. There are limited studies on these bacteria from ice and beverages that are popularly vended and consumed in Jakarta, Indonesia. In this study, we have detected and enumerated Salmonella enterica serovar Typhimurium from ice and beverages collected from several areas comprising five different regions of Jakarta, Indonesia. A total of 50 beverages and 50 ices were colected from five areas of Jakarta. Enumeration of these bacteria in both ice and beverages was determined by using threetube Most Probable Number (MPN) method, and ranged from <0.3 to > 110 MPN/ml. The highest MPN value >11000 MPN/mL were found in beverage sample B.EJ.4 and ice sample I.WJ.9. The presence of virulence genes sequences were determined by using multiplex PCR with specific genes rfbJ, fliC, and fljB. Twenty one of 424 suspected colonies (4.95%) from beverage sample and 17 of 568 suspected colonies (2.99%) from ice sample were indicated as positive isolates of S. enterica serovar Typhimurium. The results of antibiotic resistance assay were varied among all isolates. The highest percentage of resistance for isolates from beverages is 71.43% to gentamicin (10 p.g), and the lowest is 9.52% for trimethoprim (5 pg). While for isolates from ice, the highest percentage of resistance 82.35 % shown for streptomycin (10 pg) and erythromycin (15 pg), and there is no resistance isolates for trimethoprim (5 pg). The presence of Salmonella enterica serovar Typhimurium from ice and beverages in Jakarta indicate the risk of Salmonella infection. Opini Anda Klik untuk menuliskan opini Anda tentang koleksi ini! Lihat Sejarah Pengadaan  Konversi Metadata   Kembali

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