| Mobile genetic elements such as plasmids, transpososons and integrons have crucial role in spreading antibiotic resistance as well as virulence genes among bacterial populations. Integron is DNA element that can mediate the dissemination of antibiotic resistant genes or other open reading frames (ORF) within gene cassette using a site-specific recombination system. Little is known about integron and their association with antibiotic resistance and virulence genes in Indonesia. In this study ninety eight isolates of Vibrio cholerae were recovered from edible ice in Jakarta, a disc diffusion assay determined the resistance of isolates to several antibiotics. Multiplex PCR was used to detect the presence of virulence genes of V. cholerae (ctxA, ompU, tcpA, ace, zot, and toxR) as well as dissemination of class 1, 2 and 4 integron. We found that 64.29%, 60.2%, 52.04%, 38.77%, 36.73%, 19.39%, and 3.06% of the isolates were resistant to ampicillin, streptomycin, kanamycin, sulfamethoxazole-trimethoprim, erythromycin, tetracycline, and ciprofloxacin. Multiplex PCR showed the presence of toxR, ctxA, ompU, and zot genes in isolates as 75, 26, 15, and 1.3% of isolates, respectively, we did not find ace and tcpA genes in any isolates. lntegron detection performed 47% of isolates were positive for class 1 integron, 13.3% isolates carried class 2 integron, while 73.5% isolates carried class 4 integron. The presence of multidrug-resistant V. cholerae obtained from ice possessing particular virulence and class 1, 2 and 4 integron likely to be major health concern. |