Detail Edible ice in Jakarta, Indonesia, is contaminated with multidrug-resistant Vibrio cholerae with virulence potential (article of Journal of Medical Microbiology Vol.62 no.3 March 2013) Bibliografi Author: Waturangi, Diana Elizabeth ; Wennars, Melissa ; Suhartono, Magda X. ; WIJAYA, YENATA FILIA Topik: multidrug-resistant Vibrio cholerae contamination; ice used in street food; virulence potential; JABFUNG-DEW-2015-10 Bahasa: (EN )     Penerbit: Society for General Microbiology     Tempat Terbit: London, UK    Tahun Terbit: 2013     Jenis: Article - diterbitkan di jurnal ilmiah internasional Fulltext: JMM-v6n3p352full.pdf (528.5KB; 6 download) [Informasi yang berkaitan dengan koleksi ini di internet] Abstract Consumption of street food is considered a major health risk in the absence of public-health inspection programmes in Indonesia. It is hypothesized that ice used in street food could be one of the major sources of Vibrio cholerae contamination. This study documented V. cholerae contamination in edible ice from different areas of Jakarta, the capital city of Indonesia, and attempted to characterize the virulence potential of the strains. A selective medium was used to isolate 98 V. cholerae strains and their identity was confirmed using biochemical assays. Serological tests classified the majority (78?%) in the non-O1 serogroup. Multiplex PCR was used to detect the presence of V. cholerae virulence genes, namely ctxA, ompU, tcpA, ace, zot and toxR. The toxR, ctxA, ompU and zot genes were detected in 75, 26, 15 and 1?% of isolates, respectively. The ace and tcpA genes were not detected in any of the isolates. The ctxA gene encoding the cholera toxin subunit A, which has been associated only with clinical strains of O1, here was present in both serogroups. The antibiotic-resistance profile showed that 65, 60, 52, 39, 37, 19 and 3?% of the isolates were resistant to ampicillin, streptomycin, kanamycin, sulfamethoxazole–trimethoprim, erythromycin, tetracycline and ciprofloxacin, respectively. A large proportion of V. cholerae isolates came from west and south Jakarta, and these strains exhibited multidrug resistance to ampicillin, streptomycin, tetracycline, erythromycin, kanamycin and sulfamethoxazole–trimethoprim. Many of these isolates from west and south Jakarta also harboured toxR, encoding a regulator, and ctxA. The presence of multidrug-resistant V. cholerae with virulence genes in edible ice, which could cause a severe outbreak, reflects the poor water quality in Jakarta, and indicates an urgent need for better surveillance and management. Opini Anda Klik untuk menuliskan opini Anda tentang koleksi ini! Lihat Sejarah Pengadaan  Konversi Metadata   Kembali

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