ACE Gene Insertion/Deletion Polymorphism Among Patients with Type 2 Diabetes, and Its Relationship with Metabolic Syndrome at Sardjito Hospital Yogyakarta, Indonesia  

Oleh:

Sinorita, Hemi ; Madiyan, Maliyah ; Pramono, R. Bowo

Jenis: Article from Journal - ilmiah nasional - terakreditasi DIKTI
Dalam koleksi: Acta Medica Indonesiana vol. 42 no. 01 (Jan. 2010), page 12-16.
Topik: insertion/deletion polymorphism of the ACE gene; metabolic syndrome
Fulltext: 12-16.pdf (104.56KB)

Ketersediaan

Perpustakaan FK Nomor Panggil: A02.K.02 Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada

Lihat Detail Induk

Isi artikel

Aim: to know the frequencies of insertion/deletion polymorphism in the angiotensin-converting enzyme (ACE) gene among patients with type 2 diabetes and its relationship with metabolic syndrome at Sardjito Hospital Yogyakarta. Methods: we examined 69 patients with type 2 diabetes at Sardjito Hospital Yogyakarta, divided 2 groups based on ATP III criteria of metabolic syndrome. To determine the ACE genotype of the patients, a genomic DNA fragment on intron 16 of the ACE gene was amplified by polymerase chain reaction (PCR) using a forward primer 5'-CTG GAG ACC ACT CCC ATC CTT TCT-3' and reverse primer 5 '-GAT GTG GCC ATC ACA RTC GTC AGA T-3'. II genotype 1 band on 490 bp (homozigot), DD genotype 1 band on 190 bp (homozigot) and ID genotype 2 band (heterodup1ex) on 490 bp and 190 bp were separately detected on a 3% agarose gel containing ethidium bromide. Results: of 69 patients with type 2 diabetes, there were 51 females (73.91%) and 18 males (26.09%). Subjects with metabolic syndrome were 49 patients (71.02%) while without metabolic syndrome were 20 patients (28.98%). Subjects with II, DD, ID genotype were 57.97%, 23.19% and 18.84% respectively. The male subjects with II, DD, ID genotype were 55.56%, 27.78% and 16.67% respectively, and the female subject II, DD ID genotype were 58.82%, 21.57% and 19.61% respectively. The association between ACE I/D polymorphism and metabolic syndrome in type 2 diabetes, was not significant (p=0.204). Conclusion: the frequency of ACE I/D polymorphism among type 2 diabetes are 57.97% II, 23.19% DD, 18.84% ID. There is no association between metabolic syndrome and the component of metabolic syndrome and varians of the ACE gene among the type 2 diabetes patients.

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