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Antibiofilm Activity of Bacteria Isolated from Marine Environment in Indonesia against Vibrio cholerae (article of Research Journal of Microbiology Vol.6 No.12 December 2011)
Bibliografi
Author:
Waturangi, Diana Elizabeth
;
Bunardi, Y.A.
;
Magdalena, Stella
Topik:
Vibrio gallicus
;
antibiofilm activity
;
Vibrio cholerae
;
marine bacteria
;
cholera
;
JABFUNG-DEW-2015-02
Bahasa:
(EN )
Penerbit:
Academic Journals Inc.
Tempat Terbit:
New York
Tahun Terbit:
2011
Jenis:
Article - diterbitkan di jurnal ilmiah internasional
Fulltext:
RJMicrobiol v6n12 - p926-930 - text.pdf
(515.41KB;
13 download
)
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Abstract
Cholera is a waterborne-disease caused by Vibrio cholerae Biofilm inhibition has become a new approach to overcome this disease. Several publication reported marine microorganisms have been known to produce many bioactive compounds against pathogenic bacteria. The objective of this research was to obtain isolate marine bacteria with the ability to inhibit Vibrio cholerae Biofilm formation. The antibiofilm activity was determined by measuring the optical density of crystal violet which was used to stain the Vibrio cholerae Biofilm. Eleven, out of 238 isolates from several shores in Jakarta showed antibiofilm activity. Three of the eleven isolates with higher activity (KMC10, BMA1 and BLC1) were further identified using PCR and DNA sequencing of 16S rRNA gene analysis. KMC10 showed 96% similarity with Pseudoalteromonas nigrifaciens, BMA1 showed 96% similarity with Exiguobacterium sp., while BLC1 showed 99% similarity with Vibrio gallicus. Further studies are needed to characterize the bioactive compound as potential agent for cholera treatment.
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