SD DENGUE DUO® (NSl, IgG, IgM) RAPID TEST DALAM MENUNJANG DIAGNOSIS INFEKSI VIRUS DENGUE  

Oleh:

Rini, Diah Puspita ; Aryati

Jenis: Article from Journal - ilmiah nasional - terakreditasi DIKTI
Dalam koleksi: Indonesian Journal of Clinical Pathology and Medical Laboratory vol. 16 no. 02 (Mar. 2010), page 97-101.
Topik: Dengue virus infection; NS1; antidengue IgM and IgG; rapid test

Ketersediaan

Perpustakaan FK Nomor Panggil: I01.K.02 Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada

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Dengue virus infection can cause dengue hemorrhagic fever (DHF) which is still a major health problem in Indonesia. The diagnosis of DHF is established based on WHO criteria and IgM/lgG antidengue serologic markers. A rapid method is needed for the detecting or screening the disease. Antigen detection (NS1) can be performed by immunochromatography (rapid test) or enzyme -linked immunosorbent assay (ELISA). Recently, a rapid test to detect NS1 as an antigen and IgM/lgG anti dengue to differentiate primary and secondary dengue virus infection is available in one cassette. This study evaluated the new commercial dengue rapid test, SD Dengue Duo for the detection of both antigen and antibodies to dengue virus. Serum samples used in this study were collected from 33 dengue virus infection patients according to WHO criteria and admitted in the Tropical Ward, Dr. Soetomo Hospital. Samples were taken twice, during acute and convalescent phase. SD Dengue Duo (NS1, IgG, IgM) rapid test was used and confirmed by ELISA as the gold standard. To determine the diagnostic specificity 20 samples of non dengue virus infection (typhoid fever and malaria) confirmed by laboratory tests were used. In the acute phase, SD Dengue Duo (NS1, IgG, IgM) rapid test showed IgG sensitivity 94.7% (18/19), specificity 92.9% (13/14), IgM sensitivity 85% (17/20), specificity 100% (13/13), NS1 sensitivity 50% (10/20), specificity lOO% (13/13). In the convalescent phase, SD Dengue Duo (NS1, IgG, IgM) rapid test showed antidengue IgG sensitivity 96.3% (26/27), specificity 66.7% (4/6), IgM sensitivity 95% (22/23), specificity 80% (8/lO), NS1 sensitivity 42.9% (3/7), specificity lOO% (26/26). To establish the diagnosis of dengue virus infection, not only NS1, but also antidengue IgM/lgG is needed. SD Dengue Duo containing dengue NS1 antigen combined with IgG/lgM test in one cassette is a rapid, practical and has a high validity diagnostic result.

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