Bone morphogenetic protein 15 expression in human ovaries from fetuses, girls, and women  

Oleh:

Margulis, Sima ; Abir, Ronit ; Felz, Carmela

Jenis: Article from Journal - ilmiah internasional
Dalam koleksi: Fertility and Sterility (keterangan: ada di ClinicalKey) vol. 92 no. 05 (Nov. 2009), page 1666-1673.
Topik: BMP-15; human ovaries; primordial follicles; immunohistochemistry; in situ hybridization

Ketersediaan

Perpustakaan FK Nomor Panggil: F02.K.2009.04 Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada

Lihat Detail Induk

Isi artikel

Objective To investigate, for the first time, the protein expression of bone morphogenetic protein (BMP) 15 in human ovaries from fetuses, girls/women as well as its mRNA transcripts in ovaries from fetuses and girls. Design Controlled immunohistochemical and in situ hybridization study of expression of BMP-15 protein and mRNA transcripts in human ovaries. Setting Major tertiary care academic center. Patient(s) Nine patients that underwent pregnancy terminations at 21–33 gestational weeks and 18 girls and women aged 5–39 years that underwent ovarian laparoscopies. Intervention(s) None. Main Outcome Measure(s) Immunohistochemistry (protein detection) in all specimens and in situ hybridization (mRNA detection) in specimens from fetuses and girls. Both procedures were conducted on paraffin sections. Result(s) The expression of the BMP-15 protein and its mRNA was identified already from primordial stages. Protein expression was detected in all oocytes and stroma cells from both ovarian sources, and in granulosa cells of specimens from girls and women. The mRNA transcripts were detected in the oocyte, granulosa, and stroma cells from fetuses and girls. Conclusion(s) The BMP-15 protein is expressed already at primordial stages in fetuses, girls, and women, and its mRNA transcripts in fetuses and girls. Further studies should be conducted to elucidate if indeed BMP-15 is involved in the activation of human primordial follicles.

Opini Anda

Klik untuk menuliskan opini Anda tentang koleksi ini!

Kembali