Physiologic and pathologic levels of reactive oxygen species in neat semen of infertile men  

Oleh:

Desai, Nisarg ; Sharma, Rakesh ; Makker, Kartikeya

Jenis: Article from Journal - ilmiah internasional
Dalam koleksi: Fertility and Sterility (keterangan: ada di ClinicalKey) vol. 92 no. 05 (Nov. 2009), page 1626-1631.
Topik: Sperm; reactive oxygen species; chemiluminescence; sensitivity; specificity; receiver operating characteristic (ROC) curve

Ketersediaan

Perpustakaan FK Nomor Panggil: F02.K.2009.04 Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada

Lihat Detail Induk

Isi artikel

Objective To define physiologic levels of reactive oxygen species in infertile men and establish a cutoff value of reactive oxygen species level in neat semen with a high sensitivity and specificity to differentiate infertile men from fertile donors (controls). Design Reactive oxygen species levels were measured in the neat semen samples (n = 51) from fertile donors and infertile patients (n = 54). Setting Reproductive research laboratory at a tertiary care hospital. Patient(s) Infertile patients from male infertility clinic. Intervention(s) Reactive oxygen species measurement in neat semen sample using luminol-based chemiluminescence method, receiver operating characteristic curves. Main Outcome Measure(s) Seminal reactive oxygen species levels, cutoff value, sensitivity and specificity, positive and negative predictive values. Result(s) The best cutoff value to distinguish between healthy fertile donors and infertile men was 0.0185 × 106 counted photons per minute/20 × 106 sperm. At this threshold, the specificity was 82% and the sensitivity was 78%. This value can be defined as basal reactive oxygen species level in infertile men. Conclusion(s) Reactive oxygen species levels in neat semen samples as measured by luminol-based chemiluminescence are a highly specific and sensitive test in the diagnosis of infertility. This test also may help clinicians treat patients with seminal oxidative stress.

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