Improvement of in vitro culture of mouse cumulus–oocyte complexes using PDE3-inhibitor followed by meiosis induction with epiregulin  

Oleh:

Romero, Sergio ; Smitz, Johan

Jenis: Article from Journal - ilmiah internasional
Dalam koleksi: Fertility and Sterility (keterangan: ada di ClinicalKey) vol. 93 no. 03 (Feb. 2010), page 936-944.
Topik: Epiregulin; PDE3-I; IVM; follicle culture

Ketersediaan

Perpustakaan FK Nomor Panggil: F02.K.2010.01 Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada

Lihat Detail Induk

Isi artikel

Objective To explore potential helping effects of a PDE3-inhibitor (PDE3-I), Org9935, in long-term culture of cumulus–corona–oocyte complexes (COC) and to associate adequate stimuli for efficient oocyte maturation. Design Experimental in vitro study on mice. Setting Academic research laboratory. Animal(s) F1 hybrid mice: C57Bl/6j x CBA/ca. Intervention(s) The COCs were selectively retrieved from cultured follicles at the early antral stage and subcultured for 4 days in absence or presence of Org9935 (5 nM to 1 µM). Main Outcome Measure(s) Oocyte nuclear maturation; estradiol and progesterone concentrations in conditioned medium. Result(s) Survival of COCs was dose dependently improved by Org9935. When subculture was done in absence of Org9935; human chorionic gonadotropin (hCG), epiregulin, or hCG + epiregulin induced 0%, 17%, and 21%, respectively, of polar body formation. Concentrations above 50 nM prohibited the reinitiation of meiosis by a hCG + epidermal growth factor stimulus. Epiregulin or hCG + epiregulin induced 63% and 66% polar body rate, respectively, but only after washing out the inhibitor. Acute progesterone production occurs after stimulation with hCG, or hCG + epiregulin, but epiregulin alone induces the lowest response. Conclusion(s) Presence of PDE3-I in cultured COCs improves survival. Washout of PDE3-I is necessary for meiotic resumption to occur. Epiregulin by itself induces meiotic maturation but is a weak inducer of progesterone production.

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